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- Genetically modified cell lines best reflect MOA (Mechanism of Action)
- Higher activity and larger assay window for robust and reproducible cell-based bioassay
- Comprehensive application data to support assay development and validation
- Full tracible record, stringent quality control and validated cell passage stability
- Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
- Global commercial license assistance whenever regulatory filing is required
描述（Description）
The HEK293/Human ASGR1&ASGR2 Stable Cell Line was engineered to express the receptors full length human ASGR1 (Gene ID: 432) and ASGR2 (Gene ID: 433). Surface expression of human ASGR1 and ASGR2 was confirmed by flow cytometry.
应用说明（Application）
• Useful for cell-based ASGR1 or ASGR2 binding assay
生长特性（Growth Properties）
Adherent
筛选标记（Selection Marker）
Puromycin (2 μg/mL) + Hygromycin (10 μg/mL)
培养基（Complete Growth Medium）
DMEM + 10% FBS
冻存液（Freeze Medium）
Serum-free cell cryopreservation medium
装量（Quantity）
1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
存储（Storage）
Frozen in liquid nitrogen.
支原体检测（Mycoplasma Testing）
Negative
无菌检测（Sterility Testing）
Negative
使用说明（Instructions for Use）
See data sheet for detailed culturing and assay protocol.
产品数据图
Receptor Assay
Co-expression analysis of human ASGR1 and ASGR2 on HEK293/Human ASGR1&ASGR2 Stable Cell Line by FACS.
Cell surface staining was performed on HEK293/Human ASGR1&ASGR2 Stable Cell Line or negative control cell using APC-labeled anti-ASGR1 antibody and PE-labeled anti-ASGR2 antibody.
Protocol
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背景
ASGPR, a transmembrane C-type lectin, recognizes a wide variety of ligands that contain either terminal galactose (Gal) or N-acetylgalactosamine (GalNAc) residues and has been identified as a highly selective receptor on hepatocytes. The ASGR is composed of both a major (ASGR1) and minor subunit (ASGR2).
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