野生型Fc:保留天然构象,为突变体实验提供高可信度对照
突变型Fc:基于20+已上市抗体药物精准改造,直接匹配临床抗体特性
严格质控,确保批次间一致性
无内源背景干扰,数据更可靠
直接兼容FACS、ELISA、SPR 等主流检测平台
Immobilized DNP-Albumin Conjugate, Bovine at 10 μg/mL (100 μL/well) can bind Human IgG1 (L234F,L235E,P331S) Kappa Isotype Control (mAb) (Cat. No. DNP-C11P1) with a linear range of 0.2-5 ng/mL (QC tested).
Protocol
Immobilized Dinitrophenyl-BSA Conjugate (DNP-BSA) at 10 μg/mL (100 μL/well) can bind Human IgG2 (A330S, P331S) Kappa Isotype Control (mAb) (Cat. No. DNP-M884) with a linear range of 0.05-2 ng/mL (QC tested).
Protocol
Immobilized DNP-Albumin Conjugate, Bovine at 10 μg/mL (100 μL/well) can bind Monoclonal Anti-DNP antibody, Human IgG3 Isotype Control (Cat. No. DNP-M915) with a linear range of 0.1-4 ng/mL (QC tested).
Protocol
Immobilized Dinitrophenyl-BSA Conjugate (DNP-BSA) at 10 μg/mL (100 μL/well) can bind Human IgG4 (S228P, L235E) Kappa Isotype Control (mAb) (Cat. No. DNP-M886) with a linear range of 0.02-2 ng/mL (QC tested).
Protocol
2e5 of Anti-CD19 CAR-293 cells were stained with 100 μL of 1 μg/mL of Biotinylated Mouse IgG1 Isotype Control (mAb) (Cat. No. DNP-BM1) and positive control respectively, washed and then followed by PE-SA and analyzed with FACS (Routinely tested).
Protocol
2e5 of Anti-CD19 CAR-293 cells were stained with 100 μL of 1 μg/mL of FITC-Labeled Mouse IgG1 Isotype Control (mAb) (Cat. No. DNP-FM1) and positive control respectively, and FITC signal was used to evaluate the binding activity (Routinely tested).
Protocol
2e5 of anti-CD19 CAR-293 cells were stained with 100ul of 1:50 dilution of 2e5 of anti-CD19 CAR-293 cells were stained with 100ul of 1:50 dilution of Mouse IgG1 kappa Isotype Control (Cat. No. DNP-M1A1) and positive control antibody respectively, washed and then followed by PE anti-mouse IgG1 and analyzed with FACS (QC tested).
Protocol
2e5 of anti-CD19 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) Biotinylated Mouse IgG1 kappa Isotype Control (Cat. No. DNP-BM190) and positive control antibody (Cat. No. FM3-BY54) respectively, washed and then followed by PE-SA and analyzed with FACS (QC tested).
Protocol
2e5 of Anti-CD19 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of FITC-Labeled Mouse IgG1 Isotype Control (Cat. No. DNP-FM1A1) and positive control (Cat. No. FM3-FY45P1) respectively, and FITC signal was used to evaluate the binding activity (QC tested).
Protocol
5e5 of Anti-CD19 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of PE-Labeled Monoclonal Mouse IgG1 Isotype Control (Cat. No. DNP-PM1) and positive control (Cat. No. FM3-HPY53) respectively, and PE signal was used to evaluate the binding activity (QC tested).
Protocol