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分子别名（Synonym）
VTN,V75,VN,Vitronectin,S-protein,Serum-spreading factor
表达区间及表达系统（Source）
Human Truncated Vitronectin Protein, premium grade (VIN-H5117) is expressed from E. coli cells. It contains AA Val 62 - Leu 478 (Accession # P04004).
Predicted N-terminus: Met
It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.
Request for sequence蛋白结构（Molecular Characterization）
This protein carries no "tag".
The protein has a calculated MW of 47.6 kDa. The protein migrates as 46 kDa±3 kDa and 53 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
内毒素（Endotoxin）
Less than 0.01 EU per μg by the LAL method.
宿主蛋白残留（Host Cell Protein）
0.5 ng/µg of protein tested by ELISA.
宿主核酸残留（Host Cell DNA）
0.02 ng/μg of protein tested by qPCR.
无菌（Sterility）
Negative
支原体（Mycoplasma）
Negative.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Supplied as 0.2 μm filtered solution in PBS, 0.15 M Arginine, 250 mM N-Acetyl-L-cysteine, pH7.4 with glycerol as protectant.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 years under sterile conditions.
质量管理控制体系（QMS）
数据展示
电泳（SDS-PAGE）
Human Truncated Vitronectin Protein, premium grade on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-SPR
Human ITGAV&ITGB3 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT3-H52E3) immobilized on CM5 Chip can bind Human Truncated Vitronectin Protein, premium grade (Cat. No. VIN-H5117) with an affinity constant of 11.9 nM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
应用数据（Application Data）
Human Truncated Vitronectin Protein, premium grade (Cat. No. VIN-H5117) could maintain the stemness of iPSC at least Passage 5, with high expression of stem cell genes OCT4, SOX2 and NANOG in immunofluorescence.
Human Truncated Vitronectin Protein, premium grade (Cat. No. VIN-H5117) could maintain the stemness of iPSC at least Passage 5, with high expression of stem cell genes OCT4, SOX2 and SSEA4 in FACS.
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