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产品描述（Product Details）
| Assay Type | Sandwich-ELISA |
| Analyte | AAV8 |
| Format | 96T |
| Reactivity | / |
| Regulatory Status | RUO |
| Sensitivity | ≤100ng/mL |
| Standard Curve Range | 0.938 ng/mL-60 ng/mL |
| Assay Time | 3 hr 20 min |
| Suitable Sample Type | For the quantitative determination of anti-AAV8 antibodies in serum. |
| Sample volume | 100 uL |
组分（Materials Provided）
| ID | Components | Size |
| PAV008-C01 | Pre-coated AAV8 Capsid Protein Microplate | 1 plate |
| PAV008-C02 | Anti-AAV8 Antibody Standard | 2.5 μg |
| PAV008-C03 | Biotin-AAV8 Capsid Protein | 1 μg |
| PAV008-C04 | Streptavidin-HRP | 10 μg |
| PAV008-C05 | 10xWashing Buffer | 50 mL |
| PAV008-C06 | 2xDilution Buffer | 50 mL |
| PAV008-C07 | Substrate Solution | 12 mL |
| PAV008-C08 | Stop Solution | 7 mL |
产品数据图
典型数据-Typical Data Please refer to DS document for the assay protocol.
Detection of anti-AAV8 antibodies by sandwich-ELISA Assay.
Immobilized AAV8 Capsid Protein can bind Anti-AAV8 Antibody in 1:50 human serum, and then add Biotin-AAV8 Capsid Protein. Detection was performed using Streptavidin-HRP with sensitivity of 46.9 ng/mL.
The specific binding of PAV-A008 to Anti-AAV3/5/9 antibody was detected. The results showed that PAV-A008 only binds specifically to Anti-AAV8 antibody.
批内差异（Intra-Assay Statistics）
Three samples of known concentration in blank human serum were tested six times on one plate to assess intra-assay precision, Intra-Assay Precision CV≤15%.
Three samples of known concentration in blank mouse serum were tested six times on one plate to assess intra-assay precision, Intra-Assay Precision CV≤15%.
批间差异（Inter-Assay Statistics）
Three samples of known concentration in blank human serum were tested in six separate assays to assess inter-assay precision, Inter-Assay Precision CV≤15%.
Three samples of known concentration in blank mouse serum were tested in six separate assays to assess inter-assay precision, Inter-Assay Precision CV≤15%.
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