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优势特色（Features）
- Designed under ISO 9001:2015 and ISO 13485:2016
- Manufactured and QC tested under a GMP compliance factory
- FDA DMF filed
- Animal-Free materials
- Beta-lactam materials free
- Batch-to-batch consistency
- Stringent quality control tests
表达区间及表达系统（Source）
GMP Human DLL4 Protein, Fc Tag (GMP-DL4H28) is expressed from human 293 cells (HEK293). It contains AA Ser 27 - Pro 524 (Accession # NP_061947.1).
Predicted N-terminus: Ser 27
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 80.7 kDa. The protein migrates as 90 kDa±3 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 10 EU/mg by the LAL method.
蛋白A残留（Protein A）
<5 ppm of protein tested by ELISA.
宿主蛋白残留（Host Cell Protein）
<0.5 ng/µg of protein tested by ELISA.
宿主核酸残留（Host Cell DNA）
<0.02 ng/μg of protein tested by qPCR.
无菌（Sterility）
The sterility testing was performed by membrane filtration method described in CP<1101>, USP<71> and Eur. Ph. 2.6.1.
支原体（Mycoplasma）
Negative.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with protectants.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with blue ice, please inquire the shipping cost.
存储（Storage）
Upon receipt, store it immediately at -20°C or lower for long term storage.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 5 years in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
产品数据图
电泳（SDS-PAGE）
GMP Human DLL4 Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
活性（Bioactivity）-CELL BASE
GMP Human DLL4 Protein, Fc Tag (Cat. No. GMP-DL4H28) stimulates Human Notch1 (Luc) HeLa Reporter Cell. The EC50 of the effect is 9.10 µg/mL (Routinely tested).
Protocol
活性（Bioactivity）-SPR
GMP Human DLL4 Protein, Fc Tag (Cat. No. GMP-DL4H28) captured on Protein A Chip can bind Human NOTCH1 Protein, His Tag, premium grade (Cat. No. NO1-H52H3) with an affinity constant between 1.00 nM - 150 nM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
应用数据（Application Data）
CD34+ CD45+ hematopoietic cells were seeded on GMP Human DLL4 Protein, Fc Tag (Cat. No. GMP-DL4H28) - coated plates and differentiated for 26 days, then flow cytometry was used to detect the expression of T-cell progenitor markers, CD7 and CD5. GMP Human DLL4 Protein, Fc Tag (Cat. No. GMP-DL4H28) together with other growth factors could induce the formation of CD7+ and CD7+ CD5+ T-cell progenitors. However, the cells cultured without GMP Human DLL4 Protein, Fc Tag (Cat. No. GMP-DL4H28) - coated plates expressed neither CD5 nor CD7.
The Recombinant Fibronectin fragment, premium grade (Cat. No. FIN-H5116) combined with GMP Human DLL4 Protein, Fc Tag (Cat. No. GMP-DL4H28), GMP Human VCAM-1 Protein, Fc Tag (Cat. No. GMP-VC1H25) coating on the plate could efficiently induce hematopoietic stem cells differentiation to NK cells, with high expression of CD56+ CD3-.
稳定性（Stability）
The SPR based assay shows that GMP Human DLL4 Protein, Fc Tag (Cat. No. GMP-DL4H28) is stable at 37℃ for 24 hours.
The SPR based assay shows that GMP Human DLL4 Protein, Fc Tag (Cat. No. GMP-DL4H28) is stable after freezing and thawing 3 times.
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背景
Delta-like protein 4 (DLL4) is also known as Drosophila Delta homolog 4 (Delta4), which contains one DSL domain and eight EGF-like domains. DLL4 is expressed in vascular endothelium. DLL4 is involved in the Notch signaling pathway as Notch ligand, which can activates NOTCH1 and NOTCH4. DLL4 is involved in angiogenesis and negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. DLL4 can bind to Notch-1 and Notch-4.
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