APC-Labeled Human Glypican 3 / GPC3 Protein, His Tag (Site-specific conjugation)

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GP3-HA2H9-25tests
¥3520.00
GP3-HA2H9-200tests
¥21840.00
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产品信息

分子别名(Synonym)

GPC3,OCI5,Glypican-3,GTR2-2,MXR7,DGSX,SDYS ,SGB,SGBS,SGBS1

表达区间及表达系统(Source)

APC-Labeled Human Glypican 3, His Tag (GP3-HA2H9) is produced via conjugation of APC to Human Glypican 3, His Tag with a new generation site-specific technology under optimal conditions with a proprietary technology. Human Glypican 3, His Tag is expressed from human 293 cells (HEK293). It contains AA Gln 25 - His 559 (Accession # P51654-1).

Predicted N-terminus: Gln 25

Request for sequence

蛋白结构(Molecular Characterization)

Glypican 3 Structure

This protein carries a polyhistidine tag at the C-terminus

The protein has a calculated MW of 64.5 kDa.

偶联(Conjugate)

APC

Excitation Wavelength: 640 nm

Emission Wavelength: 661 nm

应用说明(Application)

Please note that this product is NOT compatible to streptavidin detection system.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, 0.5% BSA, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.

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活性(Bioactivity)-FACS

Glypican 3 FACS

5e5 of anti-GPC3 CAR-293 cells were stained with 100 μL of 1:25 dilution (4 μL stock solution in 100 μL FACS buffer) of APC-Labeled Human Glypican 3, His Tag (Cat. No. GP3-HA2H9) and negative control protein respectively. APC signal was used to evaluate the binding activity (QC tested).

Protocol

 

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背景

Glypican-3 (GPC3) is also known as Intestinal protein OCI-5, GTR2-2, MXR7, which belongs to the glypican family. Glypican 3 / GPC-3 is highly expressed in lung, liver and kidney. Glypican-3 inhibits the dipeptidyl peptidase activity of DPP4. Glypican-3 may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and also may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function.

前沿进展

 
靶点信息
  • 英文全称:

    Glypican-3

  • 中文全称:

    磷脂酰肌醇聚糖3

  • 种类:

  • 上市药物数量:

    0 详情

  • 临床药物数量:

    36 详情

  • 最高研发阶段:

    临床二期

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