收藏产品 产品信息
分子别名（Synonym）
Integrin alpha 6 beta 1,ITGA6&ITGB1
表达区间及表达系统（Source）
Human ITGA6&ITGB1 Heterodimer Protein, His Tag&Tag Free (IT1-H52W7) is expressed from human 293 cells (HEK293). It contains AA Phe 24 - Gly 1012 (ITGA6) & Gln 21 - Asp 728 (ITGB1) (Accession # P23229-2 (ITGA6) & P05556-1 (ITGB1)).
Predicted N-terminus: Phe 24 (ITGA6) & Gln 21 (ITGB1)
Request for sequence
蛋白结构（Molecular Characterization）
Human ITGA6&ITGB1 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGA6 and ITGB1, has a calculated MW of 116.8 kDa (ITGA6) and 83.7 kDa (ITGB1). Subunit ITGA6 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The non-reducing (NR) protein migrates as 140-150 kDa (ITGA6) and 100-118 kDa (ITGB1) respectively due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
产品数据图
电泳（SDS-PAGE）
Human ITGA6&ITGB1 Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
活性（Bioactivity）-ELISA
Immobilized Human ITGA6&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W7) at 5 μg/mL (100 μL/well) can bind Human Laminin 511 Protein, premium grade (Cat. No. LA8-H5283) with a linear range of 0.06-2 ng/mL (QC tested).
Protocol
Immobilized Human Laminin 511 Protein, premium grade (Cat. No. LA8-H5283) at 1 μg/mL (100 μL/well) can bind Human ITGA6&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W7) with a linear range of 0.01-0.156 μg/mL (Routinely tested).
Protocol
活性（Bioactivity）-SPR
Human ITGA6&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W7) captured on CM5 chip via anti-His antibody can bind Human Laminin 511 Protein, premium grade (Cat. No. LA8-H5283) with an affinity constant of 8.01 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
活性（Bioactivity）-BLI
Loaded Human Laminin 511 Protein, premium grade (Cat. No. LA8-H5283) on Protein A Biosensor, can bind Human ITGA6&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W7) with an affinity constant of 6.65 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
产品评论 发表评论
背景
Integrin alpha 6 beta 1, also called platelet glycoprotein GPIc-IIa, is a receptor for laminin on platelets. The ITGA6 protein product is the integrin alpha chain alpha 6. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, alpha 6 may combine with beta 4 in the integrin referred to as TSP180, or with beta 1 in the integrin VLA-6. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. ITGA6 has been shown to interact with TSPAN4 and GIPC1.
靶点信息 
数据表和文档 
联系我们 
项目合作