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分子别名（Synonym）
CSF1R,C-FMS,CD115,CSFR,FIM2,FMS,M-CSFR
表达区间及表达系统（Source）
Human M-CSF R Protein, Mouse IgG2a Fc Tag (CSR-H5255) is expressed from human 293 cells (HEK293). It contains AA Ile 20 - Glu 512 (Accession # P07333-1).
Predicted N-terminus: Ile 20
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a mouse IgG2a Fc tag at the C-terminus
The protein has a calculated MW of 81.4 kDa. The protein migrates as 105-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 0.1 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
产品数据图
电泳（SDS-PAGE）
Human M-CSF R Protein, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human M-CSF R Protein, Mouse IgG2a Fc Tag (Cat. No. CSR-H5255) is more than 85% and the molecular weight of this protein is around 210-230 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human M-CSF R Protein, Mouse IgG2a Fc Tag (Cat. No. CSR-H5255) at 5 μg/mL (100 μL/well) can bind Biotinylated Human M-CSF Protein, His,Avitag (Cat. No. MCF-H82E6) with a linear range of 0.2-3 ng/mL (QC tested).
Protocol
Immobilized Human M-CSF R Protein, Mouse IgG2a Fc Tag (Cat. No. CSR-H5255) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-34 Protein, His,Avitag (Cat. No. IL4-H82E5) with a linear range of 0.2-10 ng/mL (Routinely tested).
Protocol
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背景
Colony stimulating factor 1 receptor (CSF1R) is also known as macrophage colony-stimulating factor receptor (M-CSFR), CD115 Cluster of Differentiation 115 (CD115), C-FMS, CSFR, FIM2, FMS, and is a member of the typeⅢ subfamily of receptor tyrosine kinases (RTKs). CSF1R is a receptor for a cytokine called colony stimulating factor 1, The protein encoded by the CSFR1 gene is the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. This receptor mediates most, if not all, of the biological effects of this cytokine. Ligand binding activates CSFR1 through a process of oligomerization and transphosphorylation . Mutations in CSF1R are associated with chronic myelomonocytic leukemia and type M4 acute myeloblastic leukemia. Increased levels of CSF1R1 are found in microglia in Alzheimer's disease and after brain injuries. The increased receptor expression causes microglia to become more active. Both CSF1R, and its ligand colony stimulating factor 1 play an important role in the development of the mammary gland and may be involved in the process of mammary gland carcinogenesis.
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