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Monoclonal Anti-Monkeypox-H3L Antibody, Human IgG1 (1C5) (MALS verified)

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H3L-M611-100ug
¥3675.00
H3L-M611-1mg (500ug X 2)
¥24255.00
合计0件 产品金额¥ 0

产品详情

  • 抗体来源(Source)

    Monoclonal Anti-Monkeypox-H3L Antibody, Human IgG1 (1C5) is a chimeric monoclonal antibody recombinantly expressed from HEK293, which combines the variable region of a mouse monoclonal antibody with Human constant domain.

  • 克隆号(Clone)

    1C5

  • 亚型(Isotype)

    Human IgG1 | Human Kappa

  • 偶联(Conjugate)

    Unconjugated

  • 抗体类型(Antibody Type)

    Recombinant Monoclonal

  • 种属反应性(Reactivity)

    Virus

  • 免疫原(Immunogen)

    Recombinant Monkeypox virus (strain Zaire-96-I-16) H3L is expressed from human 293 cells.

  • 特异性(Specificity)

    Specifically recognizes Monkeypox virus (strain Zaire-96-I-16) H3L.

  • 应用(Application)

    Application
    Recommended Usage
    ELISA
    0.1-50 ng/mL 
  • 纯度(Purity)

    90% as determined by SDS-PAGE.

    90% as determined by SEC-MALS.

  • 纯化(Purification)

    Protein A purified / Protein G purified

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

数据展示

  • 电泳(SDS-PAGE)

    H3L (MPXV) SDS-PAGE

    Monoclonal Anti-Monkeypox-H3L Antibody, Human IgG1 (1C5) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    H3L (MPXV) SEC-MALS

    The purity of Monoclonal Anti-Monkeypox-H3L Antibody, Human IgG1 (1C5) (Cat. No. H3L-M611) is more than 90% and the molecular weight of this protein is around 135-165 kDa verified by SEC-MALS.

    Report
  • 活性(Bioactivity)-ELISA

     H3L (MPXV) ELISA

    Immobilized Monkeypox virus (strain Zaire-96-I-16) H3L, His Tag (Cat. No. H3L-M52H1) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Monkeypox-H3L Antibody, Human IgG1 (1C5) (Cat. No. H3L-M611) with a linear range of 0.1-2 ng/mL (QC tested).

    Protocol
  • 活性(Bioactivity)-SPR

     H3L (MPXV) SPR

    Monoclonal Anti-Monkeypox-H3L Antibody, Human IgG1 (1C5) (Cat. No. H3L-M611) captured on Protein A Chip can bind Monkeypox virus (strain Zaire-96-I-16) H3L, His Tag (Cat. No. H3L-M52H1) with an affinity constant of 0.194 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

    Protocol

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背景介绍

Monkeypox is a rare zoonosis caused by monkeypox virus, which has become the most serious orthpoxvirus and consists of complex double stranded DNA. The pathogenesis of monkeypox is that the virus invades the body from respiratory mucosa, multiplies in lymphocytes, and incurs into blood producing transient venereal toxemia. after the virus multiplies in cells, the cells can invade the blood and propagate to the skin of the whole body, causing lesions. H3L is similar to Vaccinia virus strain Copenhagen H3L heparin binding surface protein (Cop-H3L) surface membrane protein. There are research reports H3L induced transcriptional perturbations and injuries.

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