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抗体来源（Source）
Monoclonal Anti-Glycoprotein G(Lyssavirus rabies) Antibody, Human IgG1 (4A7) is a chimeric monoclonal antibody recombinantly expressed from HEK293, which combines the variable region of a mouse monoclonal antibody with Human constant domain.
克隆号（Clone）
4A7
亚型（Isotype）
Human IgG1 | Human Kappa
偶联（Conjugate）
Unconjugated
抗体类型（Antibody Type）
Recombinant Monoclonal
种属反应性（Reactivity）
Virus
免疫原（Immunogen）
Recombinant Rabies virus Glycoprotein G derived from Baculovirus-Insect cells.
特异性（Specificity）
This product is a specific antibody specifically reacts with Rabies virus Glycoprotein G.
应用（Application）
ApplicationRecommended UsageELISA0.1-75 ng/mL纯度（Purity）
90% as determined by SDS-PAGE.
90% as determined by SEC-MALS.
纯化（Purification）
Protein A purified / Protein G purified
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
数据展示
电泳（SDS-PAGE）
Monoclonal Anti-Glycoprotein G(Lyssavirus rabies) Antibody, Human IgG1 (4A7) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Monoclonal Anti-Glycoprotein G(Lyssavirus rabies) Antibody, Human IgG1 (4A7) (Cat. No. GLN-M645) is more than 90% and the molecular weight of this protein is around 135-165 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Rabies virus Glycoprotein G, His Tag (Cat. No. RAG-V55H5) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Glycoprotein G(Lyssavirus rabies) Antibody, Human IgG1 (4A7) (Cat. No. GLN-M645) with a linear range of 0.1-1 ng/mL (QC tested).
Protocol
活性（Bioactivity）-SPR
Monoclonal Anti-Glycoprotein G(Lyssavirus rabies) Antibody, Human IgG1 (4A7) (Cat. No. GLN-M645) captured on Protein A Chip can bind Rabies virus Glycoprotein G, His Tag (Cat. No. RAG-V55H5) with an affinity constant of 1.25 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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