收藏产品 产品信息
- Genetically modified cell lines best reflect MOA (Mechanism of Action)
- Higher activity and larger assay window for robust and reproducible cell-based bioassay
- Comprehensive application data to support assay development and validation
- Full tracible record, stringent quality control and validated cell passage stability
- Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
- Global commercial license assistance whenever regulatory filing is required
描述（Description）
The Human IL-31 RA/OSMR (Luc) HEK293 Reporter Cell was engineered to express STAT3 signaling response element, but also express the receptors full length human IL-31 RA (Gene ID: 133396) and OSMR (Gene ID: 9180). When stimulated with human IL-31 protein, the IL-31/IL-31 RA interaction drives STAT3-mediated luminescence. Neutralization of biological effect of human IL-31 protein by corresponding antibody results in a decrease in luminescence.
应用说明（Application）
• Screen for neutralizing antibodies blocking the stimulation of human IL-31 protein.
生长特性（Growth Properties）
Adherent
筛选标记（Selection Marker）
Puromycin (2 μg/mL) + Hygromycin (40 μg/mL) + Zeocin (20μg/mL)
培养基（Complete Growth Medium）
DMEM medium + 10% FBS
冻存液（Freeze Medium）
Serum-free cell cryopreservation medium
装量（Quantity）
1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
存储（Storage）
Frozen in liquid nitrogen.
支原体检测（Mycoplasma Testing）
Negative
无菌检测（Sterility Testing）
Negative
使用说明（Instructions for Use）
See data sheet for detailed culturing and assay protocol.
产品数据图
Receptor Assay
Co-expression analysis of human IL-31 RA and OSMR on Human IL-31 RA/OSMR (Luc) HEK293 Reporter Cell by FACS.
Cell surface staining was performed on Human IL-31 RA/OSMR (Luc) HEK293 Reporter Cell or negative control cell using PE-labeled anti-human IL-31 RA antibody and APC-labeled anti-human OSMR antibody.
Protocol
Application
Inhibition of human IL-31 protein-induced reporter activity by anti-human IL-31 RA neutralizing antibody.
This reporter cell was incubated with serial dilutions of antibodies in the presence of human IL-31 protein (Cat. No. IL1-H5247) with a final concentration of 0.001 μg/mL. The EC50 of anti-human IL-31 RA neutralizing antibody is approximately 0.609 μg/mL.
Protocol
Inhibition of human IL-31 protein-induced reporter activity by anti-human OSMR neutralizing antibody.
This reporter cell was incubated with serial dilutions of antibodies in the presence of human IL-31 protein (Cat. No. IL1-H5247) with a final concentration of 0.001 μg/mL. The EC50 of anti-human OSMR neutralizing antibody is approximately 0.727 μg/mL.
Protocol
Signaling Bioassay
Response to human IL-31 protein (RLU).
The Human IL-31 RA/OSMR (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human IL-31 protein (Cat. No. IL1-H5247). The EC50 was approximately 0.0102 μg/mL.
Protocol
Response to human IL-31 protein (FOLD).
The Human IL-31 RA/OSMR (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human IL-31 protein (Cat. No. IL1-H5247). The max induction fold was approximately 328.
Protocol
Passage Stability
Passage stability analysis by Signaling Bioassay.
The continuously growing Human IL-31 RA/OSMR (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human IL-31 protein. Human IL-31 protein stimulated response demonstrates passage stabilization (fold induction and EC50) across passage 9-20.
Protocol
产品评论 发表评论
背景
Interleukin-31 receptor subunit alpha is a protein that in humans is encoded by the IL31RA gene, also known as IL-31 receptor subunit alpha, IL-31RA, GLM-R, Gp130-like receptor, CRL3, GPL. Oncostatin M receptor (OSMR) and IL31RA form the heterodimeric receptor through which IL31 signals. IL31RA is a strong activator of STAT3 and STAT5, whereas STAT1 is only marginally tyrosine-phosphorylated. dditionally, demonstrate Jak1 binding to GPL and its activation in heteromeric complexes with the OSMR beta but also in a homomeric receptor complex.
数据表和文档 
联系我们 
项目合作