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产品参数（Product Specifications）
Assay TypeSandwich-ELISAAnalyteCas9Format96T(8×12 strips)ReactivityStreptococcus pyogenesRegulatory StatusRUOSensitivity＜0.781ng/mLStandard Curve Range0.781 ng/mL-50 ng/mLAssay Time3 hr 20 minSuitable Sample TypeFor the quantitative determination of Cas9 in Cell Therapy.Sample volume100 uL产品概述（Product Overview）
resDetect™ Cas9 (CRISPR Associated Protein 9) ELISA Kit (Residue Testing) is based on the Sandwich-ELISA method and is used for the quantitative detection of Cas9 residues in cell therapy products. It is designed to provide a reliable tool for supporting quality control in gene and cell therapy manufacturing by monitoring Cas9 residues during in-process testing and final product release, ensuring safety, and compliance in CRISPR-based therapeutic applications.
重构方法（Reconstitution）
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
存储（Storage）
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
组分（Materials Provided）
IDComponentsSizeCAS01-C01Pre-coated Anti-Cas9 Antibody Microplate1 plate(8×12 strips)CAS01-C02Cas9 Nuclease Standard20 μgCAS01-C03Biotin-Anti-Cas9 Antibody15 μgCAS01-C04Streptavidin-HRP50 μLCAS01-C0510xWashing Buffer50 mLCAS01-C062xDilution Buffer50 mLCAS01-C07Substrate Solution12 mLCAS01-C08Stop Solution7 mL质量管理控制体系（QMS）
数据展示
典型数据-Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
稀释线性（Dilution Linearity）
To assess the linearity of the assay, samples spiked with high concentrations of Cas9 nuclease were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
批内差异（Intra-Assay Statistics）
Three samples of known concentration were tested ten times on one plate to assess intra-assay precision.
批间差异（Inter-Assay Statistics）
Three samples of known concentration were tested in three separate assays to assess inter-assay precision.
回收率（Recovery）
Three Cas9 nuclease with different concentrations were tested to calculate the recovery rate.
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