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产品参数（Product Specifications）
Assay TypeSandwich-ELISAAnalyteIL-21Format96T(8×12 strips)ReactivityHumanRegulatory StatusRUOSensitivity< 1.56 pg/mLStandard Curve Range1.56 pg/mL-100 pg/mLAssay Time2 hr 50 minSuitable Sample TypeFor the quantitative determination of human IL-21 in Cell Culture Supernatants, Plasma, Serum.Sample volume100 uL产品概述（Product Overview）
resDetect™ Human Interleukin-21 (IL-21) ELISA Kit (Residue Testing) is based on the ELISA sandwich method and is used to detect and quantitatively determine GMP human IL-21 in cell culture supernatants, serum, and plasma. The kit contains GMP human IL-21 (ACROBiosystems, cat#GMP-L21H25) to ensure accurate assay results. It is designed to provide a reliable solution for CAR-T product quality assessment during drug development and CMC quality control stages. Additionally, it can be used as a universal detection tool for the quantitative determination of human IL-21.
重构方法（Reconstitution）
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
存储（Storage）
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
组分（Materials Provided）
IDComponentsSizeCRS010-C01Pre-coated Anti-IL-21 Antibody Microplate1 plate(8×12 strips)CRS010-C02Human IL-21 Standard20 μgCRS010-C03Biotin-Anti-IL-21 Antibody50 μLCRS010-C04Streptavidin-HRP50 μLCRS010-C0510xWashing Buffer50 mLCRS010-C062xDilution Buffer50 mLCRS010-C07Substrate Solution12 mLCRS010-C08Stop Solution7 mL质量管理控制体系（QMS）
数据展示
典型数据-Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
稀释线性（Dilution Linearity）
To assess the linearity of the assay, samples spiked with high concentrations of human IL-21 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
批内差异（Intra-Assay Statistics）
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision, Intra-Assay Precision CV＜10%.
批间差异（Inter-Assay Statistics）
Three samples of known concentration were tested in three separate assays to assess inter-assay precision, Inter-Assay Precision CV<10%.
回收率（Recovery）
Three parts of blank serum were added with different concentrations of human IL-21, and the serum without human IL-21 was used as background to calculate the recovery rate. The range of the recovery rate is 80.6-118.1%, and the average recovery is 94.0%.
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背景介绍
Interleukin-21 (IL-21) is a secreted protein which belongs to the IL-15 / IL-21 family. Interleukin-21 / IL-21 belongs to a family of cytokines that bind to a composite receptor consisting of a private receptor (IL21R) and the common cytokine receptor gamma chain (gamma(C)). Interleukin-21 / IL-21 impacts a number of cell types, including CD8+ memory T cells, NK cells and subsets of CD4 memory T cells. The IL-21R is widely distributed on lympho-haematopoietic cells. IL-21 is a pleiotropic cytokine produced by CD4+ T cells in response to antigenic stimulation. Its action generally enhances antigen-specific responses of immune cells. IL-21 promotes the anti-tumor activity of CD8+ T-cells and NK cells. IL-21 exerts its effect through binding to a specific type I cytokine receptor, IL-21R, which also contains the γ chain (γc) found in other cytokine receptors including IL-2, IL-4, IL-7, IL-9 and IL-15. The IL-21/IL-21R interaction triggers a cascade of events which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3.
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