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组分（Materials Provided）
IDComponentsSizeEP155-C01High-bind Plate1 plateEP155-C02Human Integrin α 4 β 725 μgEP155-C03Biotinylated Human MAdCAM-110 μgEP155-C04Anti-Integrin α 4 β 7 Neutralizing Antibody20 μgEP155-C05Streptavidin-HRP5 μgEP155-C06Coating Buffer12 mLEP155-C0710xWashing Buffer50 mLEP155-C08Blocking Buffer50 mLEP155-C09Substrate Solution12 mLEP155-C10Stop Solution7 mLEP155-C111mol/L CaCl₂1.5 mLEP155-C121mol/L MgCl₂1.5 mLEP155-C131mol/L MnCl₂1.5 mL产品概述（Product Overview）
Integrin α 4 β 7 : MAdCAM-1 [Biotinylated] Inhibitor Screening ELISA Kit is based on a competition-ELISA method, is used to screening for inhibitors of human Integrin α 4 β 7 binding to human MAdCAM-1. The kit is designed to provide a reliable solution for the screening of antibodies and small molecule inhibitors in the early stages, as well as QC release, to help researchers quickly screen and validate drugs, effectively improving research and development efficiency. It can also be used as a universal detection tool to identify the ability of human Integrin α 4 β 7 binding to human MAdCAM-1.
重构方法（Reconstitution）
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
存储（Storage）
Unopened kit should be stored at 2°C -8°C upon receiving. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
质量管理控制体系（QMS）
数据展示
典型数据-Typical Data
Please refer to DS document for the assay protocol.
INHIBITION OF Integrin α 4 β 7: MAdCAM-1 [Biotinylated] BINDING BY ANTI-Integrin α 4 β 7 NEUTRALIZING ANTIBODY
Serial dilutions of Anti-Integrin α 4 β 7 Neutralizing antibody (Catalog # EP155-C04) (1:1 serial dilution, from 5 μg/mL to 0.0098μg/mL) was added into Integrin α 4 β 7: Biotinylated MAdCAM-1 binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).
INHIBITION OF Integrin α 4 β 7: MAdCAM-1 [Biotinylated] BINDING BY selective α4 integrin antagonist
Serial dilutions of selective α4 integrin antagonist (1:1 serial dilution, from 10 μg/mL to 0.1526μg/mL) was added into Integrin α 4 β 7: Biotinylated MAdCAM-1 binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting (Routinely tested).
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