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resDetect™ Pyrophosphatase ELISA Kit

COADSMS
DS中文
DS
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RES-A005-96tests
¥6140.00
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产品详情

  • 产品参数（Product Specifications）

    Assay Type
    Sandwich-ELISA
    Analyte
    Pyrophosphatase
    Format
    96T(8×12 strips)
    Reactivity
    Human
    Regulatory Status
    RUO
    Sensitivity
    < 9.38 pg/mL
    Standard Curve Range
    9.375 pg/mL-300 pg/mL
    Assay Time
    3 hr 20 min
    Suitable Sample Type
    For the quantitative determination of Pyrophosphatase in Cell Culture Supernatants.
    Sample volume
    100 μL

  • 产品概述（Product Overview）

    resDetect™ Pyrophosphatase ELISA Kit is based on the ELISA sandwich method and is used to detect and quantitatively determine Pyrophosphatase residues in mRNA drug products or semi-manufactures. It contains Pyrophosphatase Standard (ACROBiosystems, cat# PYE-T5143) to ensure accurate assay results and is designed to provide a reliable solution for monitoring and controlling Pyrophosphatase residues during the production of mRNA preparations. It can also be used as a universal detection tool for the quantitative determination of Pyrophosphatase.

  • 重构方法（Reconstitution）

    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

  • 存储（Storage）

    1. Unopened kit should be stored at 2℃-8℃ upon receiving.

    2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

    3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • 组分（Materials Provided）

    ID
    Components
    Size
    RES005-C01
    Pre-coated Anti-Pyrophosphatase Antibody Microplate
    1 plate(8×12 strips)
    RES005-C02
    Pyrophosphatase Standard
    100 μL
    RES005-C03
    Biotin-Anti-Pyrophosphatase Antibody
    15 μg
    RES005-C04
    Streptavidin-HRP
    50 μL
    RES005-C05
    20xWashing Buffer
    50 mL
    RES005-C06
    2xDilution Buffer
    50 mL
    RES005-C07
    Substrate Solution
    12 mL
    RES005-C08
    Stop Solution
    7 mL

  • 质量管理控制体系（QMS）

    1. 质量管理体系（ISO, GMP）
    2. 质量优势
    3. 质控流程

数据展示

  • 典型数据-Typical Data

    Please refer to DS document for the assay protocol.

    Pyrophosphatase TYPICAL DATA

    For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.

验证

  • 批内差异（Intra-Assay Statistics）

    Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision, Intra-Assay Precision CV＜15%.

    Pyrophosphatase INTRA-ASSAY STATISTICS

  • 批间差异（Inter-Assay Statistics）

    Three samples of known concentration were tested in three separate assays to assess inter-assay precision, Inter-Assay Precision CV<15%.

    Pyrophosphatase INTER-ASSAY STATISTICS

  • 回收率（Recovery）

    Three Pyrophosphatase with different concentrations were tested to calculate the recovery rate.

    Pyrophosphatase RECOVERY

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背景介绍

Pyrophosphatase catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate, it can hydrolyze inorganic pyrophosphate generated with the reaction to avoid its inhibition of the reaction system. Pyrophosphatase is usually used to increase RNA yield in reverse transcription reactions. As a key raw material for in vitro transcription (IVT) of RNA, Pyrophosphatase needs to detect the residues as a protein component. This ELISA kit can be used to detect the residue of Pyrophosphatase in mRNA stock solution.

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  • 英文全称：

    Pyrophosphatases

  • 中文全称：

    焦磷酸酶类

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