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ClinMax™ Human VEGF ELISA Kit

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CEA-C068-96tests
¥3675.00
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产品详情

  • 产品概述(Product Details)

    ClinMax™ Human VEGF ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human VEGF/VEGF-A that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ClinMax™ ELISA Kit provides several benefits:

    1. Standards to calibrate with NIBSC/WHO standards for comparable results.
    2. Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
    3. High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
    4. Simplified and straightforward protocols and ready-to-use reagents to save assay time.

  • 应用说明(Application)

    The kit is developed for quantitative detection of natural and recombinant human VEGF/VEGF-A in serum, plasma and cell culture supernatants.

    It is for research use only.

  • 流程图(Workflow)

    VEGF-A Workflow

  • 关键信息(Key Features)

    Assay Type
    Sandwich-ELISA
    Analyte
    VEGF
    Format
    96-wells plate breakable into 12 x 8 wells strips
    Reactivity
    Human
    Sensitivity
    <7pg/mL
    Assay Time
    3 hr 10 min
    Sample volume
    100 uL
    Range
    31.25 pg/mL-2000 pg/mL
    Sample Type
    Cell Culture Supernatants, Plasma, Serum.
    NIBSC Code
    19/246

    Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.

  • 存储(Storage)

    Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • 组分(Materials Provided)

    ID
    Components
    Size
    CEA068-C01
    Pre-coated Anti-VEGF Antibody Microplate
    1 plate
    CEA068-C02
    Human VEGF Standard
    2000 pg×2
    CEA068-C03
    Biotin-Anti-VEGF Antibody Con. Solution
    120 μL
    CEA068-C04
    Biotin-Antibody Dilution Buffer
    15 mL
    CEA068-C05
    Streptavidin-HRP Con. Solution
    120 μL
    CEA068-C06
    Streptavidin-HRP Dilution Buffer
    15 mL
    CEA068-C07
    20× Washing Buffer
    25 mL
    CEA068-C08
    Sample Dilution Buffer
    15 mL×2
    CEA068-C09
    Substrate Solution
    15 mL
    CEA068-C10
    Stop Solution
    15 mL
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

数据展示

  • 典型数据-Typical Data

    Please refer to DS document for the assay protocol.

     VEGF-A TYPICAL DATA

    For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of VEGF is less than 7 pg/mL.

验证
  • 批内差异(Intra-Assay Statistics)

    Ten replicates of each of 3 samples containing different VEGF concentrations were tested in one assay. Acceptable criteria: CV<10%.

     VEGF-A INTRA-ASSAY STATISTICS
  • 批间差异(Inter-Assay Statistics)

    3 samples containing different concentrations of VEGF were tested in independent assays. Acceptable criteria: CV<15%.

     VEGF-A INTER-ASSAY STATISTICS
  • 回收率(Recovery)

    Recombinant VEGF was spiked into 3 human serum samples, and then analyzed. The average recovery of VEGF for serum samples is 85.87%.

     VEGF-A RECOVERY

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