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分子别名（Synonym）
4Ig-B7-H3,B7-H3,CD276,PSEC0249,UNQ309,PRO352,B7 homolog 3
表达区间及表达系统（Source）
Human B7-H3 (4Ig), His Tag (B7B-H52E7) is expressed from human 293 cells (HEK293). It contains AA Gly 27 - Thr 461 (Accession # Q5ZPR3-1).
Predicted N-terminus: Gly 27
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 48.5 kDa. The protein migrates as 65-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
产品数据图
电泳（SDS-PAGE）
Human B7-H3 (4Ig), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human B7-H3 (4Ig), His Tag (Cat. No. B7B-H52E7) is more than 85% and the molecular weight of this protein is around 55-70 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human B7-H3 (4Ig), His Tag (Cat. No. B7B-H52E7) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human B7-H3 / B7-H3 (4Ig) Antibody, Human IgG1 with a linear range of 0.3-5 ng/mL (QC tested).
Protocol
Immobilized Monoclonal Anti-Human B7-H3 / B7-H3 (4Ig) Antibody, Human IgG1 at 2 μg/mL (100 μL/well) can bind Human B7-H3 (4Ig), His Tag (Cat. No. B7B-H52E7) with a linear range of 1-10 ng/mL (Routinely tested).
Protocol
活性（Bioactivity）-BLI
Loaded Monoclonal Anti-Human B7-H3 / B7-H3 (4Ig) Antibody, Human IgG1 on Protein A Biosensor, can bind Human B7-H3 (4Ig), His Tag (Cat. No. B7B-H52E7) with an affinity constant of 1.74 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
活性（Bioactivity）-Bioactivity CELL BASE
Human B7-H3 (4Ig), His Tag (Cat. No. B7B-H52E7) inhibits Anti-CD3-induced proliferation of PBMC. The EC50 for this effect is 7.95 μg/mL (Routinely tested).
Protocol
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背景
Human B7 homolog 3 (B7-H3) is a member of the B7 family of immune proteins that provide signals for the regulation of immune responses. Other family members include B7-1, B7-2, B7-H1/PD-L1, B7-H2, and PD-L2. B7 family proteins are type I transmembrane immunoglobulin (Ig) superfamily members that contain extracellular Ig V‑like and Ig C‑like domains with a short cytoplasmic tail. Termed 4IgB7-H3 or B7-H3b, this molecule has two additional Ig-like domains (one V‑type and one C‑type) and shows a ubiquituous expression pattern.
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