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分子别名（Synonym）
CD47,MER6,IAP,OA3
表达区间及表达系统（Source）
Cynomolgus / Rhesus macaque CD47, His Tag (CD7-C52H1) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Glu 141 (Accession # F7A802-1). In the region Gln 19 - Glu 141, the AA sequence of Cynomolgus and Rhesus macaque CD47 are homologus.
Predicted N-terminus: Gln 19
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 15.8 kDa. The protein migrates as 30-50 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
Unconjugated
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
数据展示
电泳（SDS-PAGE）
Cynomolgus / Rhesus macaque CD47, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-ELISA
Immobilized Human SIRP alpha, Fc Tag (HPLC-verified) (Cat. No. SIA-H5251) at 5 μg/mL (100 μL/well) can bind Cynomolgus / Rhesus macaque CD47, His Tag (Cat. No. CD7-C52H1) with a linear range of 4-63 ng/mL (QC tested).
Protocol
Immobilized Anti-CD47, Human IgG4 at 2 μg/mL (100 μL/well) can bind Cynomolgus / Rhesus macaque CD47, His Tag (Cat. No. CD7-C52H1) with a linear range of 0.2-5 ng/mL (Routinely tested).
Protocol
活性（Bioactivity）-SPR
Anti-CD47 Mab (Human IgG4) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Cynomolgus/Rhesus macaque CD47, His Tag (Cat. No. CD7-C52H1) with an affinity constant of 1.8 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
活性（Bioactivity）-BLI
Loaded Anti-Human CD47 MAb (Human IgG4) on AHC Biosensor, can bind Cynomolgus / Rhesus macaque CD47, His Tag (Cat. No. CD7-C52H1) with an affinity constant of 2.93 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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