收藏产品 产品信息
分子别名（Synonym）
CD32a,FCGR2A,CD32,FCG2 ,FCGR2A1,IGFR2
表达区间及表达系统（Source）
Human CD32a Protein, Strep Tag (CDA-H5284) is expressed from human 293 cells (HEK293). It contains AA Ala 36 - Ile 218 (Accession # P12318-1).
Predicted N-terminus: Ala 36
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a twin strep tag at the C-terminus.
The protein has a calculated MW of 23.7 kDa. The protein migrates as 30-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
产品数据图
电泳（SDS-PAGE）
Human CD32a Protein, Strep Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human CD32a Protein, Strep Tag (Cat. No. CDA-H5284) is more than 95% and the molecular weight of this protein is around 26-38 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-SPR
Human CD32a (H167) Protein, Strep Tag (Cat. No. CDA-H5284) immobilized on CM5 Chip can bind Rituximab with an affinity constant of 1.47 μM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
Rituximab immobilized on CM5 Chip can bind Human CD32a (H167) Protein, Strep Tag (Cat. No. CDA-H5284) with an affinity constant of 1.9 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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背景
Receptors for the Fc region of IgG (Fc γ R) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Three classes of human Fc γ Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized.
There are three genes for human Fcγ RII /CD32 (A, B, and C) and one for mouse Fcγ RII B (CD32B). CD32 is a low affinity receptor for IgG. The activating isoform, CD32A, is expressed on monocytes, neutrophils, platelets and dendritic cells. CD32A is expressed on many immune cell types (macrophage, neutrophil, eosinophils, platelets, dendritic cells and Langerhan cells), where inhibitory ITIMbearing receptors may also be coexpressed and coengaged by specific ligands. CD32A delivers an activating signal upon ligand binding, and results in the initiation of inflammatory responses including cytolysis, phagocytosis, degranulation and cytokine production. The responses can be modulated by signals from the coexpressed inhibitory receptors such as CD32B, and the strength of the signal is dependent on the ratio of expression of the activating and inhibitory receptors.
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