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分子别名（Synonym）
Fc gamma RIV,CD16-2,Fcgr4
表达区间及表达系统（Source）
Biotinylated Mouse CD16-2 Protein, His,Avitag (FC4-M82E8) is expressed from human 293 cells (HEK293). It contains AA Gly 21 - Gln 203 (Accession # A0A0B4J1G0-1).
Predicted N-terminus: Gly 21
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 24.6 kDa. The protein migrates as 30-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
Biotin
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
数据展示
电泳（SDS-PAGE）
Biotinylated Mouse CD16-2 Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Biotinylated Mouse CD16-2 Protein, His,Avitag (Cat. No. FC4-M82E8) was greater than 85% as determined by SEC-HPLC.
活性（Bioactivity）-ELISA
Immobilized FMC63 at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse CD16-2 Protein, His,Avitag (Cat. No. FC4-M82E8) with a linear range of 0.3-20 ng/mL (Routinely tested).
Protocol
活性（Bioactivity）-SPR
Biotinylated Mouse CD16-2 Protein, His,Avitag (Cat. No. FC4-M82E8) captured on Biotin CAP - Series S sensor Chip can bind Herceptin with an affinity constant of 0.186 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Biotinylated Mouse CD16-2 Protein, His,Avitag (Cat. No. FC4-M82E8) captured on Biotin CAP - Series S sensor Chip can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3), premium grade (Cat. No. CDE-M120a) with an affinity constant of 31.7 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
活性（Bioactivity）-BLI
Biotinylated Mouse CD16-2 Protein, His,Avitag (Cat. No. FC4-M82E8) on SA Biosensor, can bind Rituximab with an affinity constant of 0.394 μM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Protocol
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