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分子别名（Synonym）
HMGB1,HMG1,HMG3,SBP-1
表达区间及表达系统（Source）
Human HMGB1 Protein, Mouse IgG2a Fc Tag (HM1-H5255) is expressed from human 293 cells (HEK293). It contains AA Gly 2 - Glu 215 (Accession # P09429-1).
Predicted N-terminus: Glu
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a mouse IgG2a Fc tag at the N-terminus.
The protein has a calculated MW of 51.6 kDa. The protein migrates as 55-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
Unconjugated
内毒素（Endotoxin）
Less than 0.1 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
数据展示
电泳（SDS-PAGE）
Human HMGB1 Protein, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-CELL BASE
Human HMGB1 Protein, Mouse IgG2a Fc Tag (Cat. No. HM1-H5255) stimulates production of TNF-α in Raw-246.7 cells. The EC50 for this effect is 13.18 μg/mL (Routinely tested).
Protocol
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