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分子别名（Synonym）
Integrin alpha V beta 6,ITGAV&ITGB6
表达区间及表达系统（Source）
Biotinylated Mouse ITGAV&ITGB6 Heterodimer Protein, His,Avitag&Tag Free (IT6-M82W9) is expressed from human 293 cells (HEK293). It contains AA Phe 31 - Val 988 & Gly 22 - Pro 708 (Accession # P43406-1 & Q9Z0T9-1).
Predicted N-terminus: Phe 31 | Gly 22
Request for sequence蛋白结构（Molecular Characterization）
Biotinylated Mouse ITGAV&ITGB6 Heterodimer Protein is produced by co-expression of ITGAV and ITGB6, has a calculated MW of 114.2 kDa (ITGAV) & 80.2 kDa (ITGB6). Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB6 contains no tag but a basic tail at the C-terminus.The protein migrates as 80-90 kDa and 135-150 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
Biotin
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
数据展示
电泳（SDS-PAGE）
Biotinylated Mouse ITGAV&ITGB6 Heterodimer Protein, His,Avitag&Tag Free on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%undefined.
SEC-MALS
The purity of Biotinylated Mouse ITGAV&ITGB6 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT6-M82W9) is more than 80% and the molecular weight of this protein is around 191-233 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Mouse Latent TGFB1, His Tag (Cat. No. TG1-M5245) at 2 μg/mL (100 μL/well) can bind Biotinylated Mouse ITGAV&ITGB6 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT6-M82W9) with a linear range of 1-20 ng/mL (QC tested).
Protocol
Immobilized Human Latent TGFB1, His Tag (Cat. No. TG1-H524x) at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse ITGAV&ITGB6 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT6-M82W9) with a linear range of 1-20 ng/mL (Routinely tested).
Protocol
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