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分子别名（Synonym）
Latent TGF-beta 1,Latent TGFB1,TGFB1,CED,DPD1,LAP,TGF-beta-1,TGFB
表达区间及表达系统（Source）
Cynomolgus Latent TGF-Beta 1 (C33S), His Tag (TG1-C5243) is expressed from human 293 cells (HEK293). It contains AA Leu 30 - Ser 390 (C33S) (Accession # XP_005589396.1).
Predicted N-terminus: His & Leu 280
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the N-terminus. The precursor proprotein is partially cleaved by FURIN to form mature Transforming growth factor beta-1 (TGF-beta-1) and Latency-associated peptide (LAP) chains with calculated MW of 12.7 kDa and 30.4 kDa respectively. The protein migrates as 50-60 kDa (precursor proprotein), 38-45 kDa (LAP) and 13 kDa (TGFB1) under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-HPLC.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
产品数据图
电泳（SDS-PAGE）
Cynomolgus Latent TGF-Beta 1 (C33S), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-HPLC
The purity of Cynomolgus Latent TGF-Beta 1 (C33S), His Tag (Cat. No. TG1-C5243) was greater than 90% as determined by SEC-HPLC.
活性（Bioactivity）-ELISA
Immobilized Cynomolgus Latent TGF-Beta 1 (C33S), His Tag (Cat. No. TG1-C5243) at 2 μg/mL (100 μL/well) can bind Biotinylated Human ITGAV&ITGB6 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT6-H82E4) with a linear range of 1-31 ng/mL (QC tested).
Protocol
Immobilized Cynomolgus Latent TGF-Beta 1 (C33S), His Tag (Cat. No. TG1-C5243) at 2 μg/mL (100 μL/well) can bind Biotinylated Human ITGAV&ITGB8 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT8-H82W5) with a linear range of 5-78 ng/mL (Routinely tested).
Protocol
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背景
Transforming growth factor beta 1 ( TGFB1) is also known as TGF-β1, CED, DPD1, TGFB. is a polypeptide member of the transforming growth factor beta superfamily of cytokines. It is a secreted protein that performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation and apoptosis. The TGFB1 protein helps control the growth and division (proliferation) of cells, the process by which cells mature to carry out specific functions (differentiation), cell movement (motility), and the self-destruction of cells (apoptosis). The TGFB1 protein is found throughout the body and plays a role in development before birth, the formation of blood vessels, the regulation of muscle tissue and body fat development, wound healing, and immune system function. TGFB1 is particularly abundant in tissues that make up the skeleton, where it helps regulate bone growth, and in the intricate lattice that forms in the spaces between cells (the extracellular matrix). Within cells, this protein is turned off (inactive) until it receives a chemical signal to become active. TGFB1 plays an important role in controlling the immune system, and shows different activities on different types of cell, or cells at different developmental stages. Most immune cells (or leukocytes) secrete TGFB1. TGFB1 has been shown to interact with TGF beta receptor 1, LTBP1, YWHAE, EIF3I and Decorin.
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