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Biotinylated Human Nectin-4 (244-349) Protein, His,Avitag™ (MALS verified)

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价格
Qty.
NE4-H82Ed-25ug
¥3675.00
NE4-H82Ed-200ug
¥14490.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    NECTIN4,LNIR, PRR4, PVRL4

  • 表达区间及表达系统(Source)

    Biotinylated Human Nectin-4 (244-349), His,Avitag (NE4-H82Ed) is expressed from human 293 cells (HEK293). It contains AA Phe 244 - Ser 349 (Accession # Q96NY8-1).

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    Nectin-4 Structure

    This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 15.2 kDa. The protein migrates as 18 kDa and 19-25 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 偶联(Conjugate)

    Biotin

  • 标记(Labeling)

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • 内毒素(Endotoxin)

    Less than 1.0 EU per μg by the LAL method.

  • 纯度(Purity)

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

数据展示

  • 电泳(SDS-PAGE)

    Nectin-4 SDS-PAGE

    Biotinylated Human Nectin-4 (244-349), His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • SEC-MALS

    Nectin-4 SEC-MALS

    The purity of Biotinylated Human Nectin-4 (244-349), His,Avitag (Cat. No. NE4-H82Ed) is more than 95% and the molecular weight of this protein is around 15-23 kDa verified by SEC-MALS.

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背景介绍

Nectin-4 (gene name PVRL4, poliovirus receptor-like 4) is a 66 kDa type I transmembrane glycoprotein belonging to the Nectin family of Ig superfamily proteins. Nectins are cell adhesion molecules that play a key role in various biological processes such as polarity, proliferation, differentiation and migration, for epithelial, endothelial, immune and neuronal cells, during development and adult life. Nectin-4 is a tumor-associated antigen in 50%, 49% and 86%o of breast, ovarian and lung carcinomas, respectively, mostly on tumors of bad prognosis. Its expression is not detected in the corresponding normal tissues.

前沿进展

 
药物研发进展
  • 英文全称:

    Nectin-4

  • 中文全称:

    细胞黏附分子nectin-4

  • 种类:

  • 上市药物数量:

    1 详情

  • 临床药物数量:

    14 详情

  • 最高研发阶段:

    批准上市

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