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分子别名（Synonym）
SIGLEC10,MGC126774,PRO940,Siglec10,SLG2
表达区间及表达系统（Source）
Human Siglec-10, His Tag (SI0-H52H9) is expressed from human 293 cells (HEK293). It contains AA Met 17 - Thr 546 (Accession # Q96LC7-1).
Predicted N-terminus: Met 17
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 60.1 kDa. The protein migrates as 65-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
Unconjugated
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 25 mM MES, 150 mM NaCl, Arginine, pH5.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
数据展示
电泳（SDS-PAGE）
Human Siglec-10, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human Siglec-10, His Tag (Cat. No. SI0-H52H9) is more than 90% and the molecular weight of this protein is around 132-160 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human Siglec-10, His Tag (Cat. No. SI0-H52H9) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human siglec-10 Antibody, Human IgG1 with a linear range of 0.08-2 ng/mL (QC tested).
Protocol
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