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分子别名（Synonym）
DEE41, EAAT2, EIEE41, GLT-1, GLT1, HBGT
表达区间及表达系统（Source）
Human SLC1A2 Protein, Flag,His Tag (SL2-H52D6) is expressed from human 293 cells (HEK293). It contains AA Ala 2 - Lys 574 (Accession # P43004).
Predicted N-terminus: Met
Request for sequence蛋白结构（Molecular Characterization）
This protein carries flag tag at the N-terminus and polyhistidine tag at the C-terminus.
The protein has a calculated MW of 66.0 kDa. The protein migrates as 55-70 KDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
Unconjugated
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
This product is not suitable for cell based experiments due to cytotoxicity of DDM.
DDM and CHS are INDISPENSABLE to keep membrane protein soluble and active, under no circumstances should you remove DDM and CHS.
DDM/CHS buffer (DC-11) is sold separately and not included in protein, and please contact us if you need the buffer.
If glycerol is not compatible with your application, remove glycerol just before the immediate experiment, and NEVER store glycerol-free protein solution.Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, DDM, CHS, pH7.5 with glycerol as protectant.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
质量管理控制体系（QMS）
- *The DDM/CHS buffer (Cat. No. DC-11) is sold separately and not included in protein, you can follow this link for product information.
产品展示
电泳（SDS-PAGE）
Human SLC1A2 Protein, Flag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-ELISA
Immobilized Human SLC1A2 Protein, Flag,His Tag (Cat. No. SL2-H52D6) at 10 μg/mL (100 μL/well) can bind Monoclonal Anti-Human SLC1A2 antibody, Mouse IgG with a linear range of 0.156-1.25 μg/mL (QC tested).
Protocol
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