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Human TGF-beta RIII / TGFBR3 Protein, His Tag

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Qty.
TG3-H52H3-100ug
¥3675.00
TG3-H52H3-1mg (500ug X 2)
¥24255.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    BGCAN, betaglycan

  • 表达区间及表达系统(Source)

    Human TGF-beta RIII / TGFBR3 Protein, His Tag (TG3-H52H3) is expressed from human 293 cells (HEK293). It contains AA Gly 21 - Val 787 (Accession # Q03167-1).

    Predicted N-terminus: Gly 21 

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    TGF-beta RIII Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 86.7 kDa. The protein migrates as 100 kDa and 130-170 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 偶联(Conjugate)

    Unconjugated

  • 内毒素(Endotoxin)

    Less than 1.0 EU per μg by the LAL method.

  • 纯度(Purity)

    >90% as determined by SDS-PAGE.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

数据展示

  • 电泳(SDS-PAGE)

    TGF-beta RIII SDS-PAGE

    Human TGF-beta RIII / TGFBR3 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • 活性(Bioactivity)-BLI

     TGF-beta RIII BLI

    Loaded Biotinylated Human TGF-Beta 1, Avitag (Cat. No. TG1-H8217) on SA Biosensor, can bind Human TGF-beta RIII / TGFBR3 Protein, His Tag (Cat. No. TG3-H52H3) with an affinity constant of 1.80 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).

    Protocol

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背景介绍

This locus encodes the transforming growth factor (TGF)-beta type III receptor. The encoded receptor is a membrane proteoglycan that often functions as a co-receptor with other TGF-beta receptor superfamily members. Ectodomain shedding produces soluble TGFBR3, which may inhibit TGFB signaling. Decreased expression of this receptor has been observed in various cancers. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.

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